Brazil were held to a 1-1 draw by Panama in a friendly at Estadio do Dragao on Saturday.
The five-times world champions played poorly and were unable dominate the Central Americans, ranked 76th in the world.
Milan's Lucas Paqueta gave Brazil the lead in the 32nd minute, but captain Adolfo Machado found the equaliser just four minutes later.
At a post-match press conference, Brazil head coach Tite could not hide his disappointment, calling it a "bad result".
Opposition manager Julio Dely Valdes was keen to emphasise the historic importance of the match, Panama's first goal and draw against the South American giants.
Brazil now head to Prague where they will face the Czech Republic on Tuesday.
The double-header against Panama and the Czech Republic are Tite's last tests before he names his squad for the Copa America in June.