A tanker left the eastern Libyan port of Ras Lanuf on Wednesday carrying the first oil export from the terminal in two years, a company official said.
The tanker is loaded with 776,000 barrels of crude oil to be delivered to Italy, Omar al-Fetouri, a company official told Libya 24 news broadcaster.
Another tanker is being prepared to be shipped soon.
Libya's state-owned National Oil Corporation (NOC) announced last week that exports would resume from the eastern region of Oil Crescent after forces aligned with an administration based in the eastern city of Tobruk regained four major oil terminals in the region from a rival militia.
The terminals were seized around two years ago amid the anarchy that hit Libya after the overthrow of longtime dictator Moamer Gaddafi in a 2011 uprising.
Libya's income heavily relies on revenues from oil exports that have dropped from 1.6 million barrels a day in the final days of Gaddafi's reign to 200,000 barrels every day.
In March, the UN-backed unity government arrived in Tripoli amid international hopes it would be able to re-establish stability and stop the spread of Islamic State extremists in the country.
However, Haftar and the Tobruk-based elected parliament have refused to recognize the Tripoli government.